Use of whole blood in the measurement of neutrophil migration.

The use of dried whole blood absorbed on filter-paper for the Evaluation of Diphtheria and Tetanus Antitoxins in Mass Surveys

NEUTROPHIL CHEMOTACTIC DEFECT IN 60 PATIENTS WITH RICKETS

Neutrophil mobility in 60 patients with vitamin D-deficient rickets is studied, and the patients are followed after therapy by 600,000 units vitamin D. 28 of 60 patients (46%), had low chemotaxis for neutrophils before vitamin D injection (migration), and 36 of 60 (60%) had low random mobility. The results showed a significant improvement after vitamin D therapy furthermore, the level of c...

Microfluidic kit-on-a-lid: a versatile platform for neutrophil chemotaxis assays.

Improvements in neutrophil chemotaxis assays have advanced our understanding of the mechanisms of neutrophil recruitment; however, traditional methods limit biologic inquiry in important areas. We report a microfluidic technology that enables neutrophil purification and chemotaxis on-chip within minutes, using nanoliters of whole blood, and only requires a micropipette to operate. The low sampl...

DEFECTIVE NEUTROPHIL MOBILITY IN TEN PATIENTS WITH VITAMIN D-DEFICIENT RICKETS

We studied the immune system, especially the chemotactic activity of neutrophils, in ten patients with vitamin D-deficient rickets and compared the results with ten healthy controls of matched age. Among all immune system factors, the chemotactic studies persistantly showed remarkable deficiency in leukocyte mobility, both random motion and migration. When compared with controls, the findi...

PAPILLON-LEFE\'VRE SYNDROME: NEUTROPHIL MOTILITY AND KILLING DEFECT IN A CHILD WITH RECURRENT SEVERE INFECTIONS

A case of palmoplantar hyperkeratosis with periodontosis and a history of recurrent severe pyoderma, pneumonia and multiple liver abscesses is described in a 12 year old girl. The patient demonstrated neutrophil dysfunction characterized by decreased random migration and chemotaxis and defective bactericidal activity. The exact immunopathological mechanism for susceptibility to infections i...